NRAS Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.29 kDa and the accession number is P01111.

Human

E. coli

NS6,NRAS1,N-ras,neuroblastoma RAS viral (v-ras) oncogene homolog,NCMS,CMNS,ALPS4

Met1-Cys186

> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM.