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LAIR1 Protein, Rhesus macaque, Recombinant (His)

产品编号 TMPJ-00427

LAIR1 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-33 KDa and the accession number is A0A0U3SBU1.

LAIR1 Protein, Rhesus macaque, Recombinant (His)

LAIR1 Protein, Rhesus macaque, Recombinant (His)

产品编号 TMPJ-00427
LAIR1 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-33 KDa and the accession number is A0A0U3SBU1.
规格价格库存数量
10 μg
¥ 550
5日内发货
50 μg
¥ 1,670
5日内发货
500 μg
¥ 13,700
5日内发货
1 mg
¥ 20,500
5日内发货
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常见问题解答
化合物带有盐酸盐离子、硫酸盐离子等是否和其本身有什么区别?盐形式和游离态有什么区别?
盐和非盐形式化合物的活性分子是一样的,在生物实验中起到的效果也一致,活性和使用方法都是一样的。只是由于呈盐不同,物理性质比如溶解度会有差异。建议您根据溶解、实验需求进行选择。
如何选择某个靶点的特异性或总的抑制剂?特异性和非特异性的区别是什么?
抑制剂按照特异性分为广谱 pan 和特异性 selective 两种。Pan 为某个靶点总的抑制剂,对所有亚型或整个家族的成员都有抑制作用。Selective 抑制剂针对某个蛋白激酶的某个亚型或家族中某个成员抑制率特别高或有特异性抑制作用。 一般评价一个抑制剂的抑制效率主要看 IC50 值,IC50 值越低,说明抑制剂效率越高。建议您根据以上几个特征进行综合选择,也可联系技术帮您推荐相关抑制剂。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
LAIR1 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-33 KDa and the accession number is A0A0U3SBU1.
种属
Rhesus
表达系统
HEK293 Cells
标签C-6xHis
蛋白编号A0A0U3SBU1
别名
Leukocyte-Associated Immunoglobulin-Like Receptor 1,LAIR-1,LAIR1,hLAIR1,CD305
氨基酸序列
Gln22-Try165
蛋白构建
Gln22-Try165
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量25-33 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.2.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1) is a single-pass type I membrane protein. LAIR1 expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells, highly in naive T-cells and B-cells. As an inhibitory receptor, LAIR1 plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. LAIR1 plays inhibitory role independently of SH2-containing phosphatases and modulates cytokine production in CD4+ T-cells. It down-regulates IL2 and IFNG production while inducing secretion of transforming growth factor beta, also down-regulates IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. LAIR1 inhibits the differentiation of peripheral blood precursors towards dendritic cells. It also restrains proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells.

参考文献

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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