IL-4 Protein, Mouse, Recombinant

IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.

规格	价格/CNY		数量
5 μg	￥ 510	现货
10 μg	￥ 810	5日内发货
20 μg	￥ 1,320	5日内发货
50 μg	￥ 2,480	5日内发货
100 μg	￥ 4,010	5日内发货
200 μg	￥ 7,110	5日内发货
500 μg	￥ 15,100	5日内发货

规格

价格/CNY

货期

数量

5 μg

￥ 510

现货

10 μg

￥ 810

5日内发货

20 μg

￥ 1,320

5日内发货

50 μg

￥ 2,480

5日内发货

100 μg

￥ 4,010

5日内发货

200 μg

￥ 7,110

5日内发货

500 μg

￥ 15,100

5日内发货

生物活性	Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-4 ng/mL.
产品描述	IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.

种属	Mouse
表达系统	E. coli
标签	Tag Free
蛋白编号	P07750
别名	白介素, 白细胞介素, BSF-1, Il-4, interleukin 4
蛋白构建	The Mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	13.4 kDa (predicted)

内毒素	Please contact us for more information.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy