IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 820 | 现货 | ||
50 μg | ¥ 2,480 | 5日内发货 | ||
500 μg | ¥ 12,100 | 5日内发货 | ||
1 mg | ¥ 17,400 | 5日内发货 |
生物活性 |
1. Loaded Cynomolgus IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.33 nM as determined in BLI assay. (Regularly tested).
2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.5 ng/ml. (QC verified);
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产品描述 | IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-6xHis |
蛋白编号 | P05112 |
别名 | Pitrakinra, Binetrakin, B-Cell Stimulatory Factor 1, IL4, Interleukin-4, IL-4, 白介素, BSF-1, Lymphocyte Stimulatory Factor 1, 白细胞介素 |
氨基酸序列 | His25-Ser153 |
蛋白构建 | His25-Ser153 |
蛋白纯度 |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
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分子量 | 18 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response. |
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IL-4 Protein, Human, Recombinant (His) Pitrakinra IL 4 Binetrakin B-Cell Stimulatory Factor 1 IL4 Interleukin-4 IL-4 BSF 1 白介素 BSF-1 BSF1 Lymphocyte Stimulatory Factor 1 白细胞介素 recombinant recombinant-proteins proteins protein