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IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)

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货号 TMPK-00161

别名 Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta

Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) is expressed in E. coli expression system with C-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P01584.

IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)

IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)

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Rating icon 很棒
SPR兼容缓冲液
货号 TMPK-00161 别名 Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta

Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) is expressed in E. coli expression system with C-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P01584.

规格价格库存数量
5 μg
¥ 313
现货
10 μg
¥ 498
3日内发货
20 μg
¥ 793
3日内发货
50 μg
¥ 1,580
3日内发货
100 μg
¥ 2,660
现货
200 μg
¥ 4,790
5日内发货
500 μg
¥ 10,600
5日内发货
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选择批次:
纯度: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
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产品介绍


生物活性
产品描述
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) is expressed in E. coli expression system with C-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P01584.
生物活性
1. Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA (QC Test). 2. Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay.
IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)
研究背景
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
种属
Human
表达系统
E. coli
标签C-His-Avi
蛋白编号P01584
蛋白构建Ala117-Ser269
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta
内毒素< 1 EU/μg by the LAL method.
化学信息
分子量20.1 kDa (predicted) same as Tris-Bis PAGE result.
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

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剂量转换

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