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CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)
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还可以货号 TMPY-05580
别名 NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.
其他形式的 “CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)”:
CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)
一键复制产品信息 还可以货号 TMPY-05580 别名 NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 428 | 6-8日内发货 | |
| 10 μg | ¥ 683 | 6-8日内发货 | |
| 20 μg | ¥ 1,130 | 5日内发货 | |
| 50 μg | ¥ 2,270 | 5日内发货 | |
| 100 μg | ¥ 4,430 | 5日内发货 | |
| 200 μg | ¥ 6,730 | 5日内发货 | |
| 500 μg | ¥ 13,800 | 5日内发货 | |
| 1 mg | ¥ 28,800 | 5日内发货 |
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| 产品描述 | CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1. |
| 生物活性 | Immobilized Human CD73, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6 ng/ml determined by ELISA (QC Test). |
| 研究背景 | CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-hFc |
| 蛋白编号 | P21589-1 |
| 蛋白构建 | Trp27-Lys547 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73) |
| 内毒素 | Less than 1EU per μg by the LAL method. |
| 分子量 | 84.5 kDa (Predicted); 85-100 kDa (Due to glycosylation) |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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