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CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1.

| 规格 | 价格 | 库存 | 数量 |
|---|
| 生物活性 | Immobilized Biotinylated Human CD73, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5 ng/ml determined by ELISA. |
| 产品描述 | CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His-Avi |
| 蛋白编号 | P21589-1 |
| 蛋白构建 | Trp27-Lys547 |
| 蛋白纯度 | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| 分子量 | 60.92 kDa (Predicted); 60-70 kDa (Due to glycosylation) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases. |