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CD4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 43.6 kDa and the accession number is P01730.

| 规格 | 价格 | 库存 | 数量 | 
|---|---|---|---|
| 5 μg | ¥ 246 | 6-8日内发货 | |
| 10 μg | ¥ 383 | 6-8日内发货 | |
| 20 μg | ¥ 616 | 5日内发货 | |
| 50 μg | ¥ 1,190 | 5日内发货 | |
| 100 μg | ¥ 1,990 | 5日内发货 | |
| 200 μg | ¥ 3,590 | 5日内发货 | |
| 500 μg | ¥ 8,000 | 5日内发货 | 
| 生物活性 | 1. Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. 
2. Immobilized Human CD4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 10.2ng/ml / 10.6ng/ml / 10.6ng/ml determined by ELISA. | 
| 产品描述 | CD4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 43.6 kDa and the accession number is P01730. | 
| 种属 | Human | 
| 表达系统 | HEK293 Cells | 
| 标签 | C-His-Avi | 
| 蛋白编号 | P01730 | 
| 别名 | EU3,CD4mut,CD4 receptor,CD4 molecule,CD4 antigen (p55),CD4 | 
| 蛋白构建 | Lys26-Trp390 | 
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC | 
| 分子量 | 43.6 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. | 
| 内毒素 | < 1 EU/μg by the LAL method. | 
| 蛋白性状 | Lyophilized powder | 
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. | 
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. | 
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. | 
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. | 
| 研究背景 | CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |