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CD45 Protein, Human, Recombinant (His & Avi), Biotinylated

CD45 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00424
别名: LCA, LY5, EC 3.1.3.48, CD45, L-CA, T200, CD45R, PTPRC, B220, GP180

PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. CD45 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 47.3 kDa and the accession number is P08575-4.

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CD45 Protein, Human, Recombinant (His & Avi), Biotinylated
规格 价格/CNY 货期 数量
100 μg ¥ 5,180 5日内发货
500 μg ¥ 20,700 5日内发货
1 mg ¥ 34,600 5日内发货
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产品目录号及名称: CD45 Protein, Human, Recombinant (His & Avi), Biotinylated (TMPK-00424)
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生物活性
技术参数
产品性质
参考文献
生物活性 Immobilized Anti-CD45 Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0.16μg/ml determined by ELISA.
产品描述 PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. CD45 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 47.3 kDa and the accession number is P08575-4.
种属 Human
表达系统 HEK293 Cells
标签 C-His-Avi
蛋白编号 P08575-4
别名 LCA, LY5, EC 3.1.3.48, CD45, L-CA, T200, CD45R, PTPRC, B220, GP180
蛋白构建 Gln26­-Lys416
蛋白纯度 > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量 47.3 kDa (predicted). Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
内毒素 < 1 EU/μg by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.

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Keywords

CD45 Protein, Human, Recombinant (His & Avi), Biotinylated T 200 CD 45 B-220 LCA LY 5 LY5 T-200 EC 3.1.3.48 CD45 GP 180 L-CA CD-45 B 220 T200 CD45R LY-5 PTPRC B220 GP180 GP-180 recombinant recombinant-proteins proteins protein

 

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