CD45R0 Protein, Human, Recombinant (His)

Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.

规格	价格/CNY		数量
10 μg	￥ 750	5日内发货
50 μg	￥ 2,260	5日内发货
500 μg	￥ 12,800	5日内发货
1 mg	￥ 19,200	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 750

5日内发货

50 μg

￥ 2,260

5日内发货

500 μg

￥ 12,800

5日内发货

1 mg

￥ 19,200

5日内发货

产品描述

种属	Human
表达系统	Human Cells
标签	C-6His
蛋白编号	P08575-4
别名	CD45 antigen, L-CA, protein tyrosine phosphatase, receptor type, C, CD45, PTPRC, B220, LY5, receptor-type tyrosine-protein phosphatase C, CD45R, LCA
氨基酸序列	Gln26Lys416
蛋白构建	Recombinant Human Receptor-type Tyrosine-protein Phosphatase C is produced by our Mammalian expression system and the target gene encoding Gln26Lys416 is expressed with a 6His tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	80-120 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.

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CD45R0 Protein, Human, Recombinant (His)

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