CD45 Protein, Human, Recombinant (His & Avi)

PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.

规格	价格/CNY		数量
100 μg	￥ 3,100	5日内发货
500 μg	￥ 12,400	5日内发货
1 mg	￥ 20,700	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 3,100

5日内发货

500 μg

￥ 12,400

5日内发货

1 mg

￥ 20,700

5日内发货

生物活性

Immobilized Human CD45, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.

产品描述

种属	Human
表达系统	HEK293
标签	C-His-Avi
蛋白编号	P08575-4
别名	CD45R, LY5, EC 3.1.3.48, B220, CD45, T200, L-CA, GP180, LCA, PTPRC
蛋白构建	Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln26-Ile416.[Accession \|P08575-4]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.