Tubulin-IN-59 (Compound 8g) is an orally effective microtubule (Tubulin) inhibitor. It exhibits potent antiproliferative activity against HCT116 colorectal cancer, HeLa cervical cancer, and HepG2 liver cancer cell lines, with IC₅₀ values ranging from 16-35 nM. Tubulin-IN-59 arrests HeLa cells in the G2/M phase and induces apoptosis. It also demonstrates significant antitumor activity in the HT29 human colon cancer mouse xenograft model. Tubulin-IN-59 is applicable for research on colorectal and cervical cancers.