Topoisomerase I inhibitor 4 (compound 7a) is a potent inhibitor of topoisomerase I activity. It effectively inhibits the proliferation of various cancer cell lines including HepG2, A549, MCF-7, and HeLa, with IC50 values of 1.20 μM, 2.09 μM, 1.56 μM, and 1.92 μM, respectively. Thus, Topoisomerase I inhibitor 4 holds promise for cancer research applications [1].

Topoisomerase I inhibitor 4 (1-80 μM) shows Top1 inhibitory activity [1]. Topoisomerase I inhibitor 4 (0-40 μM; 48 h) inhibits proliferation of cancer cells [1]. Cell Proliferation Assay [1] Cell Line: HepG2, A549, MCF-7 and HeLa cell lines Concentration: 0-40 μM Incubation Time: 48 hours Result: Showed antiproliferative activities to HepG2, A549, MCF-7 and HeLa cells with IC 50 s of 1.20, 2.09, 1.56 and 1.92 μM, respectively.