Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Topoisomerase I inhibitor 2 是一种高度选择性的DNA 拓扑异构酶 I(Top1) 抑制剂,可抑制Top1活性并导致 DNA 损伤。Topoisomerase I inhibitor 2 阻断 G2/M 期并诱导细胞凋亡,具有抗肿瘤作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 6-8周 | ||
50 mg | ¥ 13,800 | 6-8周 | ||
100 mg | ¥ 17,500 | 6-8周 |
产品描述 | Topoisomerase I inhibitor 2 (ZML-8) is a highly selective DNA topoisomerase I (Top1) inhibitor that inhibits Top1 activity and results DNA damage. Topoisomerase I inhibitor 2 blocks G2/M phase and induces apoptosis with anti-tumor effect [1]. |
体外活性 | Topoisomerase I inhibitor 2 (24 hours) exhibits strong inhibition with an IC 50 value of 0.58 μM towards HepG2 and selective activity with SI values (selectivity index) of 55.70% between HepG2 and normal human liver cell line L-02 [1]. Topoisomerase I inhibitor 2 (1.25, 2.5 μM, 48 hours) inhibits tumor cells proliferation by decreasing anti-apoptotic expression of Bcl-2 and enhancing caspase-dependent apoptosis [1]. Topoisomerase I inhibitor 2 (1.25, 2.5 μM, 48 hours) decreases Top1 specific activity and results in DNA damage by causing supercoiled DNA [1]. Cell Cycle Analysis [1] Cell Line: HepG2 cells Concentration: 2.5 μM Incubation Time: 24 hours Result: Arrested the cell cycle in G2/M phase in a dose-dependent manner. Cell Proliferation Assay [1] Cell Line: HepG2 cells Concentration: 0.625, 1.25, 2.5 μM Incubation Time: 48 hours Result: Inhibited tumor cells proliferation by inducing cell apoptosis in a dose-dependent manner, the apoptosis rate was 65.0% and 77.6%, respectively. Western Blot Analysis [1] Cell Line: HepG2 cells Concentration: 0.625, 1.25, 2.5 μM Incubation Time: 48 hours Result: Decreased anti-apoptotic expression of Bcl-2 at 2.5 μM significantly and increased the expression of pro-apoptotic protein Bax, Bad, and p53. And also significantly enhanced caspase- dependent apoptosis and activated Cleaved caspase-3 in a dose-dependent manner. |
分子量 | 293.32 |
分子式 | C18H15NO3 |
CAS No. | 2588211-44-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Topoisomerase I inhibitor 2 2588211-44-1 Inhibitor inhibitor inhibit