Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Topoisomerase I/II inhibitor 2 (compound 1a) 可显著抑制小鼠模型中的异种移植肿瘤的生长,可用于肝癌治疗。Topoisomerase I/II inhibitor 2 是一种DNA 拓扑异构酶 I/II 的双重抑制剂。Topoisomerase I/II inhibitor 2 是有效的拓扑异构酶抑制剂,对LM9 细胞和Huh7 细胞的 IC50值分别为 6.83 μM 和9.82 μM 。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 10-14周 | ||
50 mg | ¥ 13,800 | 10-14周 | ||
100 mg | ¥ 17,500 | 10-14周 |
产品描述 | Topoisomerase I/II inhibitor 2 (compound 1a) can significantly reduce the xenograft tumor growth in mice model which can be used in treating liver cancer. Topoisomerase I/II inhibitor 2 is a DNA topoisomerase I/II dual inhibitor. Topoisomerase I/II inhibitor 2 is a potent Topoisomerase inhibitor with IC50 values of 6.83 μM and 9.82 μM for LM9 cells and Huh7 cells [1]. |
体外活性 | Topoisomerase I/II inhibitor 2 (compound 1a) (0-150 μM; 72 hours) has favourable anti-proliferative activity in cancer cell lines, and better inhibitory activity on two human hepatocellular carcinoma cell lines (HuH7, LM9) [1]. Topoisomerase I/II inhibitor 2 (20 μM; 24 hours) has no damage to the DNA of HuH7 cells while some damage is noticed on LM9 cells [1]. Topoisomerase I/II inhibitor 2 (1.25-8 μM; 1-2 weeks) inhibits cell proliferation of LM9 and Huh7 in a concentration-dependent manner [1]. Topoisomerase I/II inhibitor 2 (1.25-8 μM; 24 hours) has a good inhibitory effect on the migration and invasion of LM9 and HuH7 cells with concentration-dependent manner [1]. Topoisomerase I/II inhibitor 2 (0-20 μM; 24 hours) can inhibit the expression of matrix metalloproteinases-9 (MMP-9) in LM9 and HuH7 cells [1]. Topoisomerase I/II inhibitor 2 (0-20 μM; 48 hours) inhibits cells proliferation by blocking cell cycle at the G 2 /M phase [1]. Topoisomerase I/II inhibitor 2 (3.5-20 μM; 48 hours) can injure mitochondrial function and induce cell apoptosis in a concentration-dependent manner [1]. Cell Proliferation Assay Cell Line: LM9, HuH7, SK-hep-1, HepG2, HT-29, HCT-116, RKO, SW480, MCF-7, MDA-B-231, HGC-27, SGC-7901, BGC-823, A549, U251, HL-60, LO2 [1] Concentration: 0-150 μM Incubation Time: 72 hours Result: Displayed favourable anti-proliferative activity and had better inhibitory activity on two human hepatocellular carcinoma cell lines (HuH7, LM9). Western Blot Analysis Cell Line: LM9 and HuH7 cells [1] Concentration: 0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 Incubation Time: 48 hours Result: Inhibited the expression of MMP-9. Cell Cycle Analysis Cell Line: LM9 and HuH7 cells [1] Concentration: 0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 Incubation Time: 48 hours Result: Inhibited cells proliferation by blocking cell cycle at the G 2 /M phase. Apoptosis Analysis Cell Line: LM9 and HuH7 cells [1] Concentration: 3.5, 7, 14 μM in LM9; 5, 10, 20 μM in HuH7 Incubation Time: 48 hours Result: Induced apoptosis in a dose-dependent manner. |
分子量 | 336.34 |
分子式 | C19H16N2O4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Topoisomerase I/II inhibitor 2 Inhibitor inhibitor inhibit