Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RRD-251 是一种(Rb)-Raf-1相互作用抑制剂,可诱导转移性黑色素瘤细胞凋亡并能与达卡巴嗪协同作用,具有抗增殖、抗血管生成和抗肿瘤活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 252 | 5日内发货 | ||
5 mg | ¥ 413 | 5日内发货 | ||
10 mg | ¥ 722 | 5日内发货 |
产品描述 | RRD-251 is an Rb-Raf-1 interaction inhibitor that induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine[1]. |
体外活性 | RRD-251 (10-50 μM; 24 hours) inhibits melanoma growth in-vitro [1]. RRD-251 (50 μM; 2 hours) inhibits Rb-Raf-1 interaction and Rb phosphorylation in non-small cell lung cancer cells [1]. RRD-251 induces apoptosis (50 μM; 18 hours) and cell cycle arrest (20-50 μM; 4 hours) [1]. RRD-251 alters the expression of cell cycle and apoptosis regulatory protein [1]. Cell Proliferation Assay [1] Cell Line: SK-MEL-28 cells, SK-MEL-5 cells, SK-MEL-2 cells Concentration: 10 μM, 20 μM, 50 μM Incubation Time: 24 hours Result: Inhibited melanoma growth in-vitro. Western Blot Analysis [1] Cell Line: SK-MEL-28 cells, SK-MEL-5 cells, SK-MEL-2 cells Concentration: 50 μM Incubation Time: 2 hours Result: Showed the depletion of phosphorylated-Rb in RRD-251 treated cells. Apoptosis Analysis [1] Cell Line: SK-MEL-28 cells, SK-MEL-5 cells, SK-MEL-2 cells Concentration: 50 μM Incubation Time: 18 hours Result: Induced apoptosis. Cell Cycle Analysis [1] Cell Line: SK-MEL-28 cells, SK-MEL-5 cells Concentration: 20 μM, 50 μM Incubation Time: 4 hours Result: Resulted in a dose dependent inhibition of cell cycle progression in SK-MEL-28 and SK-MEL-5 cells, respectively. |
体内活性 | RRD-251 (50 mg/kg; i.p.; q.o.d; for 14 days) has anti-cancer activities in vivo on melanomas [1]. Animal Model: 8-wk-old female athymic nude mice, with SK-ME-28 xenograft [1] Dosage: 50 mg/kg Administration: Intraperitoneal administration, qod, for 14 days Result: Inhibits the growth of SK-ME-28 xenograft in nude mice. |
分子量 | 271.59 |
分子式 | C8H9Cl3N2S |
CAS No. | 72214-67-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RRD-251 72214-67-6 Inhibitor inhibitor inhibit