R-1479 (4'-Azidocytidine) is an RdRp inhibitor with an IC50 value of 1.28 μM for HCV RNA replication in the HCV subgenomic replicon system.

HCV replication:1.28 μM

The membrane-associated, native HCV replicase complex is isolated from 2209-23 HCV replicon cells and a derived cell line carrying HCV replicon RNA with an S282T mutation in the NS5B coding sequence. The in vitro replicase assay contain 10 μL of cytoplasmic membrane fraction, 50 mM HEPES (pH 7.5), 10 mM KCl, 10 mM dithiothreitol, 5 mM MgCl2, 20 μg/mL actinomycin D, 1 mM ATP, 1 mM GTP, 1 mM UTP, 30 μCi of [α-33P]CTP (3000 Ci/mmol, 10 mCi/mL), 1 unit/μL SUPERase•In, 10 mM creatine phosphate, and 200 μg/mL creatine phosphokinase in a final volume of 25 μL [1].

4'-叠氮基胞嘧啶核苷, 4'-Azidocytidine

DMSO: 90 mg/mL (316.64 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.61 mM), Sonication is recommended.