M464 is a potent and orally active non-steroidal anti-inflammatory compound functioning as an NLRP3 inflammasome inhibitor. It works by preventing ASC oligomerization and decreasing ROS production, thereby inhibiting pyroptosis and blocking the expression of downstream Caspase-1 and the release of IL-1β. M464 demonstrates protective effects against acute lung and liver injury in mice and can be utilized in research on NLRP3-related inflammatory diseases.