规格

价格

库存

数量

100 mg

¥ 131

现货

500 mg

¥ 280

现货

1 g

¥ 338

现货

5 g

¥ 800

现货

1 mL x 10 mM (in DMSO)

¥ 338

现货

生物活性

产品描述	IPTG (Isopropyl β-D-thiogalactoside) is a synthetic, non-metabolizable galactose analog. IPTG can trigger the transcription process of the lactose operon, thereby inducing the expression of corresponding proteins encoded by downstream genes of the lactose operon.
体外活性	方法：携带 PLtetO-1-luc 报告质粒的大肠杆菌加入 IPTG：浓度梯度（0.01-5 mM），培养至对数生长期，检测萤光素酶（luciferase）活性，计算表达水平变化倍数。结果：Plac/ara-1 受 IPTG 诱导，调控范围达 1800 倍。[1]
激酶实验	For RNA interference (RNAi), A549 cells are plated at 1,500 cells per well in 96-well plates, allowed to adhere for 24 hours, and transfected with 25 nM siRNA oligonucleotide using Dharmafect 4. Transfected cells are treated with the indicated concentrations of GNE-617 (0.1, 1 , 10 , 100 , and 1000 nM) for 72 hours and viability is evaluated with CellTiter-Glo. Lysates for detection of NAPRT1 protein are collected 72 hours after transfection of 1 million A549 cells in 10 cm dishes. For NAPRT1 re-expression, RERF-LC-MS cells are transfected with pCMV6-AC.NAPRT1 and empty vector pCMV6-AC using Amaxa Nucleofector technology and selected with Geneticin
别名	异丙基-beta-D-硫代半乳糖吡喃糖苷, Isopropyl β-D-thiogalactoside

化学信息

分子量	238.3
分子式	C₉H₁₈O₅S
CAS No.	367-93-1
Smiles	S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
密度	1.37 g/cm3

储存&溶解度

存储

Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

DMSO: 237 mg/mL (994.54 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (20.98 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1964 mL	20.9820 mL	41.9639 mL	209.8196 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL	41.9639 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL	20.9820 mL
20 mM	0.2098 mL	1.0491 mL	2.0982 mL	10.4910 mL
50 mM	0.0839 mL	0.4196 mL	0.8393 mL	4.1964 mL
100 mM	0.0420 mL	0.2098 mL	0.4196 mL	2.0982 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

IPTG

IPTG

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

IPTG

IPTG

IPTG 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词