ETX1975-3 is an orally active inhibitor and bactericide targeting cytochrome bd oxidase in Mycobacterium tuberculosis. It disrupts electron transfer between the b-heme centers of the target enzyme and, when used with Q203, exhibits bactericidal activity against both replicating and non-replicating Mycobacterium tuberculosis, reducing bacterial load in acute mouse models. ETX1975-3 retains activity against multidrug-resistant/extensively drug-resistant clinical isolates of Mycobacterium tuberculosis and non-tuberculous mycobacteria, while also demonstrating favorable preclinical ADMET properties. This compound is applicable in research concerning tuberculosis and non-tuberculous mycobacterial infections.