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Anti-TNF-alpha Antibody (1Q810)
还可以产品编号 TMAY-03728
Anti-TNF-alpha Antibody (1Q810) 是一种 Rabbit 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody (1Q810) 可用于 WB,ICC/IF。
Anti-TNF-alpha Antibody (1Q810)
Anti-TNF-alpha Antibody (1Q810)
还可以产品编号 TMAY-03728
Anti-TNF-alpha Antibody (1Q810) 是一种 Rabbit 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody (1Q810) 可用于 WB,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-TNF-alpha Antibody (1Q810) is a Rabbit antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (1Q810) can be used in WB,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1Q810 |
| 交叉反应 | Rat |
| 验证活性 | 1. Immunofluorescence staining of rat TNFα in rat spleen cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-rat TNFα monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane. 2. Anti-TNFalpha rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Rat spleen tissue Lysate. -Lane C: Rat brain tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:25 kDa. -Observed band size:30 kDa |
| 应用 | WB,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Rat TNF-alpha (rR TNF-alpha; TMPY-02202; P16599; Leu80-Leu235). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Rat TNF-alpha Protein (TMPY-02202) |
| 抗原种属 | Rat |
| 研究领域 | Cancer Drug Targets, Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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