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别名 NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Anti-NT5E Antibody (1N164) 是一种 Rabbit 抗体,靶向 NT5E。Anti-NT5E Antibody (1N164) 可用于 IHC,WB。
Anti-NT5E Antibody (1N164) 是一种 Rabbit 抗体,靶向 NT5E。Anti-NT5E Antibody (1N164) 可用于 IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-NT5E Antibody (1N164) is a Rabbit antibody targeting NT5E. Anti-NT5E Antibody (1N164) can be used in IHC,WB. |
| 别名 | NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73) |
| Ig Type | IgG |
| 克隆号 | 1N164 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of CD73 on A431 cells lysates using anti-CD73 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD73 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD73 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CD73 antibody. Counter stained with hematoxylin. ![]() ![]() ![]() ![]() |
| 应用 | IHCWB |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 70 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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