Anti-MRSA agent 38 is a broad-spectrum antibacterial compound (MIC= 0.0625-2 µg/mL) that inhibits ribosomal protein synthesis. It exerts multiple bactericidal actions by disrupting bacterial membrane structure and inducing ROS accumulation. Furthermore, Anti-MRSA agent 38 selectively targets tumor cells, such as HGC-27 (IC50= 0.86 µM), MRC-5 (IC50= 5.52 µM), and RPC (IC50= 6.09 µM) cells. This agent is applicable in research on bacterial infections and other infectious diseases.