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Anti-HER2/ERBB2 Antibody (8K444) 是一种 Mouse 抗体,靶向 HER2/ERBB2。Anti-HER2/ERBB2 Antibody (8K444) 可用于 IHC,IF,WB。
Anti-HER2/ERBB2 Antibody (8K444) 是一种 Mouse 抗体,靶向 HER2/ERBB2。Anti-HER2/ERBB2 Antibody (8K444) 可用于 IHC,IF,WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-HER2/ERBB2 Antibody (8K444) is a Mouse antibody targeting HER2/ERBB2. Anti-HER2/ERBB2 Antibody (8K444) can be used in IHC,IF,WB. |
别名 | TKR1, NGL, NEU, MLN19, MLN 19, HERVW, HERV7Q, herstatin, HER-2, HER2, ERVWE1, ERBB2, ENVW, ENV, EGFR2, CD340 |
Ig Type | IgG2a, Kappa |
克隆号 | 8K444 |
交叉反应 | Human |
验证活性 | 1. Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2(ABT088) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7, Predicted band size: 137kDa, Observed band size: 200kDa. 2. Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2(ABT088) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: SK-BR-3, Predicted band size: 137kDa, Observed band size: 200kDa. 3. Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2(ABT008) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: T47D, Predicted band size: 137kDa, Observed band size: 180kDa. 4. Human breast carcinoma tissue was stained with Anti-Her-2 (ABT008) Antibody. 5. Human breast carcinoma tissue was stained with Anti-Her-2 (ABT008) Antibody. 6. Human metastatic hepatocellular carcinoma of the breast tissue was stained with Anti-Her-2 (ABT008) Antibody. |
应用 | |
推荐剂量 | IHC: 1:100-500 IF: 1:100-500 WB: 1:500-2000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
性状 | Liquid |
缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
研究背景 | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. |
偶联 | Unconjugated |
免疫原 | A synthesized peptide: human Her-2 |
抗原种属 | human |
基因ID | |
Uniprot ID |
分子量 | Theoretical: 137 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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