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Anti-HER2/ERBB2 Antibody (3F246)
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产品编号 TMAB-00847
Anti-HER2/ERBB2 Antibody (3F246) 是一种 Mouse 抗体，靶向 HER2/ERBB2。Anti-HER2/ERBB2 Antibody (3F246) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-HER2/ERBB2 Antibody (3F246)
Anti-HER2/ERBB2 Antibody (3F246)
还可以产品编号 TMAB-00847
Anti-HER2/ERBB2 Antibody (3F246) 是一种 Mouse 抗体，靶向 HER2/ERBB2。Anti-HER2/ERBB2 Antibody (3F246) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-HER2/ERBB2 Antibody (3F246) is a Mouse antibody targeting HER2/ERBB2. Anti-HER2/ERBB2 Antibody (3F246) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 3F246 |
| 交叉反应 | Human |
| 验证活性 | 1. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: SW480 (Human) Cell Lysate at 30 μg Lane 3: Siha (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-C-erbB-2/HER2 (TMAB-00847) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 185/100-110 kDa Observed band size: 185 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C-erbB-2/HER2) Monoclonal Antibody, conjugated (TMAB-00847) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Sample: Lane 1: Human A431 cell lysates Lane 2: Human LOVO cell lysates Lane 3: Human HeLa cell lysates Primary: Anti-C-erbB-2/HER2 (TMAB-00847) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 138 kDa Observed band size: 180 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus.Isoform 2: Cytoplasm. Nucleus.Isoform 3: Cytoplasm. Nucleus. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]. |
| 免疫原 | Recombinant Protein: human HER2 protein |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | EGF,Oncoproteins,Tumor-associated antigens,EGF,Receptor Tyrosine Kinases,Tumor Associated |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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