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Anti-CYP1A1 Polyclonal Antibody
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产品编号 TMAB-00522
Anti-CYP1A1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CYP1A1。Anti-CYP1A1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CYP1A1 Polyclonal Antibody
Anti-CYP1A1 Polyclonal Antibody
还可以产品编号 TMAB-00522
Anti-CYP1A1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CYP1A1。Anti-CYP1A1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CYP1A1 Polyclonal Antibody is a Rabbit antibody targeting CYP1A1. Anti-CYP1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Pig) |
| 验证活性 | 1. Sample: K562 Cell (Human) Lysate at 30 μg Primary: Anti-CYP1A1 (TMAB-00522) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 2. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-CYP1A1 (TMAB-00522) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP1A1) Polyclonal Antibody, Unconjugated (TMAB-00522) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Endoplasmic reticulum |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human CYP1A1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Drug metabolism,Cancer,Cytochromes,Hypoxia,Lipases,Drug metabolism,Cytochromes,Response to hypoxia,Cellular metabolic process |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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