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Anti-AK3L1 Antibody (7P613)

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Anti-AK3L1 Antibody (7P613)
产品编号 TMAY-02330
别名 AK4, AK3L2, AK3L1, AK3, adenylate kinase 4

Anti-AK3L1 Antibody (7P613) 是一种 Rabbit 抗体,靶向 AK3L1。Anti-AK3L1 Antibody (7P613) 可用于 ELISA,IP。

Anti-AK3L1 Antibody (7P613)
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Anti-AK3L1 Antibody (7P613)

Rating icon 还可以
Anti-AK3L1 Antibody (7P613)
产品编号 TMAY-02330 别名 AK4, AK3L2, AK3L1, AK3, adenylate kinase 4

Anti-AK3L1 Antibody (7P613) 是一种 Rabbit 抗体,靶向 AK3L1。Anti-AK3L1 Antibody (7P613) 可用于 ELISA,IP。

规格价格库存数量
100 μL
¥ 2,289
5日内发货
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产品介绍

生物活性
产品描述
Anti-AK3L1 Antibody (7P613) is a Rabbit antibody targeting AK3L1. Anti-AK3L1 Antibody (7P613) can be used in ELISA,IP.
别名AK4, AK3L2, AK3L1, AK3, adenylate kinase 4
Ig Type
Rabbit IgG
克隆号
7P613
交叉反应
Human
验证活性
AK4 was immunoprecipitated using:
-Lane A:0.5 mg MCF-7 Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate.
-Lane C:0.5 mg 293T Whole Cell Lysate.
-Lane D:0.5 mg A431 Whole Cell Lysate.
-2 µL anti-AK4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-AK4 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 26 kDa.
-Observed band size: 26 kDa
应用
ELISA,IP
推荐剂量
ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human AK4 / AK3L1 (rh AK4 / AK3L1; TMPY-04469; P27144-1; Ala2-Tyr223).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human AK4 / AK3L1 protein (TMPY-04469)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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