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Anti-AK3L1 Antibody (3I126)
还可以产品编号 TMAY-02329
Anti-AK3L1 Antibody (3I126) 是一种 Rabbit 抗体，靶向 AK3L1。Anti-AK3L1 Antibody (3I126) 可用于 WB,ICC/IF,IP。
Anti-AK3L1 Antibody (3I126)
Anti-AK3L1 Antibody (3I126)
还可以产品编号 TMAY-02329
Anti-AK3L1 Antibody (3I126) 是一种 Rabbit 抗体，靶向 AK3L1。Anti-AK3L1 Antibody (3I126) 可用于 WB,ICC/IF,IP。
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抗原信息
| 产品描述 | Anti-AK3L1 Antibody (3I126) is a Rabbit antibody targeting AK3L1. Anti-AK3L1 Antibody (3I126) can be used in WB,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3I126 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. AK4 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-AK4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-AK4 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa. 3. Anti-AK4 rabbit monoclonal antibody at 1:500 dilution. -Lane A: MCF7 Whole Cell Lysate. -Lane B: HepG2 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa |
| 应用 | WB,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human AK4 / AK3L1 (rh AK4 / AK3L1; TMPY-04469; P27144-1; Ala 2-Tyr 223). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human AK4 / AK3L1 protein (TMPY-04469) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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