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Anti-ACTA1 Antibody (8R189)
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还可以货号 TMAC-00077
别名 Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Anti-ACTA1 Antibody (8R189) 是一种 Rabbit 抗体，靶向 ACTA1。Anti-ACTA1 Antibody (8R189) 可用于 ICC/IF,IHC,IP,WB。
Anti-ACTA1 Antibody (8R189)
Anti-ACTA1 Antibody (8R189)
一键复制产品信息 还可以货号 TMAC-00077 别名 Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Anti-ACTA1 Antibody (8R189) 是一种 Rabbit 抗体，靶向 ACTA1。Anti-ACTA1 Antibody (8R189) 可用于 ICC/IF,IHC,IP,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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| 产品描述 | Anti-ACTA1 Antibody (8R189) is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Antibody (8R189) can be used in ICC/IF,IHC,IP,WB. |
| 别名 | Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA |
| Ig Type | IgG |
| 克隆号 | 8R189 |
| 反应种属 | Human,Mouse,Rat,zebrafish |
| 验证活性 | 1. Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish, Lane 2: Hela, Lane 3: A431, Lane 4: Jurkat. 2. Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue, Lane 2:Hybrid fish (crucian-carp) kidney tissue. 3. Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin. 5. ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.        |
| 应用 | ICC/IFIHCIPWB |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 42 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |
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