规格

价格

库存

数量

50 mg

¥ 28,900

10-14周

100 mg

¥ 43,300

10-14周

200 mg

¥ 65,000

10-14周

生物活性

产品描述	ADU-S100 (MIW815) disodium salt is an activator of stimulator of interferon genes (STING).
体外活性	ADU-S100 shows enhanced type I IFN production over CDA in THP-1 human monocytes. ADU-S100 induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. ADU-S100 is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). ADU-S100 induces significantly higher levels of IFN-α when compared to ML cGAMP.
体内活性	ADU-S100 shows higher anti-tumor control than the endogenous ML cGAMP. A dose-response of the ADU-S100 compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicits maximum tumor antigen-specific CD8+ T cell responses, and improves long-term survival to 50%.
细胞实验	Cryopreserved hPBMCs are thawed and 1×10^6 cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO2. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer.
动物实验	WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm3 they received three IT doses of ADU-S100 at 5, 25, 50, or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17, and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C).
别名	ML RR-S2 CDA disodium salt, MIW815 disodium salt

化学信息

分子量	734.51
分子式	C₂₀H₂₂N₁₀Na₂O₁₀P₂S₂
CAS No.	1638750-95-4
Smiles	[Na].[Na].[H][C@@]12COP(S)(=O)O[C@@]3([H])[C@@H](O)[C@@]([H])(O[C@]3([H])COP(S)(=O)O[C@]([H])([C@@H]1O)[C@@]([H])(O2)n1cnc2c(N)ncnc12)n1cnc2c(N)ncnc12jcExt:v:0:0,1:0
密度	1.31g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: 300 mg/mL (408.44 mM), Sonication is recommended.

溶液配制表

H2O

	1mg	5mg	10mg	50mg
1 mM	1.3615 mL	6.8073 mL	13.6145 mL	68.0726 mL
5 mM	0.2723 mL	1.3615 mL	2.7229 mL	13.6145 mL
10 mM	0.1361 mL	0.6807 mL	1.3615 mL	6.8073 mL
20 mM	0.0681 mL	0.3404 mL	0.6807 mL	3.4036 mL
50 mM	0.0272 mL	0.1361 mL	0.2723 mL	1.3615 mL
100 mM	0.0136 mL	0.0681 mL	0.1361 mL	0.6807 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

ADU-S100 disodium salt

ADU-S100 disodium salt

产品介绍

引用文献

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体内实验配液计算器

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关键词

ADU-S100 disodium salt

ADU-S100 disodium salt

ADU-S100 disodium salt 相关产品

产品介绍

引用文献

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体内实验配液计算器

剂量转换

关键词