ethynylcytidine

Ethynylcytidine属于核苷类似物，是 RNA 合成过程的高效抑制剂，可对 RNA 聚合酶 Ⅰ、Ⅱ、Ⅲ 发挥抑制作用。Ethynylcytidine在多种癌症模型中均展现出显著的抗肿瘤活性，同时还是一类重要的点击化学试剂；其分子结构中含有炔基，能够与叠氮基修饰的分子发生铜催化的叠氮 - 炔环加成反应（CuAAc）。

2'-beta-C-Ethynylcytidine is a Nucleoside Derivative - 2'-Modified nucleoside.

5'-Ethynyl-2'-deoxycytidine (EdC) functions as a nucleoside analog effectively inhibiting the replication of herpes simplex virus-1 (HSV-1) KOS strain with an ID50 of 0.2 μg/mL. Additionally, it decreases virus-induced cytopathogenic effects in PRK cells for HSV-1, HSV-2, and vaccinia virus strains, demonstrating MIC values between 0.2 to 5 μg/mL. EdC acts as a thymidylate synthetase inhibitor, selectively impacting the DNA incorporation rates of [1',2'-3H]deoxyuridine compared to [CH3-3H]deoxythymidine in PRK cells with ID50s of 3 and 120 μg/mL, respectively. It further inhibits thymidine synthetase and curtails L1210 cell proliferation, a process reversible by deoxythymidine (ID50s are 4.4 and 1,000 μg/mL, respectively). Also, EdC is utilized in monitoring DNA synthesis and cellular replication through click chemistry conjugation, linking its ethynyl group with the azido group of various fluorochromes.