Palmitoyl alkyne-coenzyme A (palmitoyl alkyne-CoA), a thioester formed from ω-alkynylated palmitic acid and CoA, serves as a chemical reporter for the labeling of palmitoylated proteins through click chemistry. This process utilizes probes such as biotin-azide or 3-azido-7-hydroxycoumarin for both in vitro and in vivo studies.