UCHL1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-02175

UCHL1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q9R0P9.

UCHL1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-02175

UCHL1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q9R0P9.

规格	价格	库存	数量
100 μg	¥ 3,170	5日内发货

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产品信息

生物活性	Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
产品描述	UCHL1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q9R0P9.
种属	Mouse
表达系统	E. coli
标签	N-His
蛋白编号	Q9R0P9
别名	UCHL-1,UCH-L1,ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase),R75593,PGP9.5,gad,C88048,AW822034
蛋白构建	A DNA sequence encoding the mouse UCHL1 (Q9R0P9) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
蛋白纯度	> 97 % as determined by SDS-PAGE
分子量	26.2 kDa (predicted); 29 kDa (reducing conditions)
内毒素	Please contact us for more information.
缓冲液	Supplied as sterile 50 mM Tris, 150 mM NaCl, 20% glycerol, pH 7.7.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.