Transferrin R/CD71 Protein, Human, Recombinant (hFc)

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. Transferrin R/CD71 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 101.8 kDa and the accession number is P02786-1.

规格	价格/CNY		数量
100 μg	￥ 2,210	5日内发货
500 μg	￥ 8,900	5日内发货
1 mg	￥ 14,800	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,210

5日内发货

500 μg

￥ 8,900

5日内发货

1 mg

￥ 14,800

5日内发货

生物活性

1. Immobilized Human Transferrin R, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA (QC Test). 2. Human Transferrin R, hFc Tag captured on CM5 Chip via Anti-Fc Antibody can bind Human Transferrin, His Tag with an affinity constant of 0.1μM as determined in SPR assay.

产品描述

种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	P02786-1
别名	sTfR, CD71, TfR1, TFR, p90, TFRC, TR, T9
蛋白构建	Cys89-Phe760
蛋白纯度	> 95% as determined by Tris-Bis PAGE
分子量	101.8 kDa (predicted). Due to glycosylation, the protein migrates to 105-115 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.