TNF RII Protein, Human, Recombinant (hFc)

TNF RII Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 60-90 KDa and the accession number is P20333.

规格	价格/CNY		数量
10 μg	￥ 740	5日内发货
50 μg	￥ 1,970	5日内发货
500 μg	￥ 12,100	5日内发货
1 mg	￥ 17,400	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 740

5日内发货

50 μg

￥ 1,970

5日内发货

500 μg

￥ 12,100

5日内发货

1 mg

￥ 17,400

5日内发货

生物活性

1. Loaded Recombinant Human TNF RII (C-Fc) on Pro-A Biosensor, can bind Recombinant Human TNF alpha with an affinity constant of 25.2 pM as determined in BLI assay. (Regularly tested) 2. Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RII inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha. The IC50 for this effect is 2.5 ng/mL. (Regularly tested) 3. Immobilized Recombinant Human TNF alpha at 5μg/ml (100 μl/well) can bind Recombinant Human TNF RII (C-Fc) . The ED50 of Recombinant Human TNF RII (C-Fc) is 15.35 ng/ml. (Regularly tested)

产品描述

TNF RII Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 60-90 KDa and the accession number is P20333.

种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	P20333
别名	TNF-R2, Tumor necrosis factor receptor superfamily member 1B, TNF-RII, TNFRSF1B, Tumor necrosis factor receptor 2, p75, p80 TNF-α receptor, p80 TNF-alpha receptor, CD120b, Tumor necrosis factor receptor type II
氨基酸序列	Leu23-Asp257
蛋白构建	Leu23-Asp257
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	60-90 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.

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TNF RII Protein, Human, Recombinant (hFc)

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