TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 560 | 现货 | ||
20 μg | ¥ 810 | 5日内发货 | ||
50 μg | ¥ 1,340 | 5日内发货 | ||
100 μg | ¥ 1,950 | 现货 |
生物活性 |
1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can TNF-α with an affinity constant of 0.2843 nM as determined in a SPR assay(Routinely tested).
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产品描述 | TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375. |
种属 | Human |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P01375 |
别名 | TNFA, TNFSF2, TNF-alpha, TNF-α, DIF, 肿瘤坏死因子, tumor necrosis factor |
蛋白构建 | The Human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus. |
蛋白纯度 |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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分子量 | 17.5 kDa (predicted) |
内毒素 | < 5 EU/mg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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TNF alpha Protein, Human, Recombinant TNFA TNFSF2 TNFSF-2 TNF-alpha TNF-α DIF 肿瘤坏死因子 TNFSF 2 tumor necrosis factor recombinant recombinant-proteins proteins protein