TNF alpha 重组蛋白, Human | 肿瘤坏死因子

TNF alpha Protein, Human, Recombinant

产品编号 TMPY-00936

TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.

规格	价格	库存
10 μg	¥ 560	现货
20 μg	¥ 810	现货
50 μg	¥ 1,340	现货
100 μg	¥ 1,950	现货

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常见问题解答

问如何选择合适的溶剂？

答常用的溶剂包括DMSO、水、乙醇等。建议您根据抑制剂给出的溶解度参考，选择合适的溶剂。如果该抑制剂可以溶于DMSO，建议使用没有吸潮新开封的 DMSO，如果有潮气污染，很容易带来抑制剂降解或难溶的问题。如果该抑制剂可以溶于水，建议您根据实验类型选择无菌水、生理盐水、无菌 PBS 、培养基等作为溶剂。

问超声一般需要多久？

答超声时长和溶解的量和浓度都有关，一般建议半个小时左右，如果浓度接近最大溶解度，或者需溶解的粉末较多，建议延长超声时间，并辅以加热。

问产品的溶解度是多少？

答建议您先查询TargetMol官网、陶术官网，看该抑制剂的产品介绍，找到溶解度信息。如果没有相应信息，建议联系技术对该化合物的溶解度进行测定。

问加药后检测结果没有作用，是不是产品质量有问题？

答产品质量没有问题，都是经过两次严格质检的，HNMR确定产品结构，HPLC确定化合物纯度。同一个抑制剂由于实验材料（细胞、动物）的不同，IC50 和实验效果也是不一样的，不能照搬文献报道的方法，应该根据自己的实验设立浓度梯度，得到一个最适合的浓度。

问PEG300 可以用 PEG400 替代吗？

答PEG300 具有适度的粘稠度，有利于药物的溶解和给药过程，同时也能够提供良好的耐受性，不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议，因为熔点接近常温。

产品信息

生物活性	1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. 2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can TNF-α with an affinity constant of 0.2843 nM as determined in a SPR assay(Routinely tested).
产品描述	TNF alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.5 kDa and the accession number is P01375.
种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	P01375
别名	肿瘤坏死因子,tumor necrosis factor,TNF-α,TNFSF2,TNF-alpha,TNFA,DIF
蛋白构建	A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus. Predicted N terminal: Met
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	17.5 kDa (predicted); 17.5 kDa (reducing conditions)
内毒素	< 5 EU/mg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

TNF alpha Protein, Human, Recombinant

TNF alpha Protein, Human, Recombinant

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