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TNF alpha Protein, Human, Recombinant (aa 77-233, His)

TNF alpha Protein, Human, Recombinant (aa 77-233, His)

产品编号 TMPJ-00124
别名: TNF-a, TNF-α, Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF α, Cachectin, TNF, TNFSF2, TNF-Alpha, 肿瘤坏死因子

TNF alpha Protein, Human, Recombinant (aa 77-233, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16 KDa and the accession number is P01375.

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TNF alpha Protein, Human, Recombinant (aa 77-233, His)
规格 价格/CNY 货期 数量
10 μg ¥ 340 5日内发货
50 μg ¥ 820 5日内发货
500 μg ¥ 4,700 5日内发货
1 mg ¥ 6,720 5日内发货
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产品目录号及名称: TNF alpha Protein, Human, Recombinant (aa 77-233, His) (TMPJ-00124)
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生物活性
技术参数
产品性质
生物活性 Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-40 pg/ml. (QC verified)
产品描述 TNF alpha Protein, Human, Recombinant (aa 77-233, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16 KDa and the accession number is P01375.
种属 Human
表达系统 E. coli
标签 C-6xHis
蛋白编号 P01375
别名 TNF-a, TNF-α, Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF α, Cachectin, TNF, TNFSF2, TNF-Alpha, 肿瘤坏死因子
氨基酸序列 Val77-Leu233
蛋白构建 Val77-Leu233
蛋白纯度 Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量 16 KDa (reducing condition)
内毒素 < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons, IL2, and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8, Lys9, Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.

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Keywords

TNF alpha Protein, Human, Recombinant (aa 77-233, His) TNF-a TNFSF 2 TNF-α Tumor Necrosis Factor TNFA TNFSF-2 Tumor Necrosis Factor Ligand Superfamily Member 2 TNF α Cachectin TNF TNFSF2 TNF-Alpha 肿瘤坏死因子 recombinant recombinant-proteins proteins protein

 

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