TNF alpha Protein, Human, Recombinant (His & Avi)

TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375.

规格	价格/CNY		数量
100 μg	￥ 2,210	5日内发货
500 μg	￥ 8,900	5日内发货
1 mg	￥ 14,800	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,210

5日内发货

500 μg

￥ 8,900

5日内发货

1 mg

￥ 14,800

5日内发货

生物活性	1. Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA. 2. Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay.
产品描述	TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375.

种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P01375
别名	TNFα, APC1 protein, 肿瘤坏死因子, TNFSF2, TNF, TNF α, TNF-alpha, TNF-α, TNFSF1A, TNFATNF, TNFalpha, Cachectin, DIF, TNFA
蛋白构建	Val77-Leu233
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	20.3 kDa (predicted). Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.