TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.

规格	价格/CNY		货期	数量
20 μg	￥ 5,730	5日内发货
100 μg	￥ 15,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 5,730

5日内发货

100 μg

￥ 15,800

5日内发货

生物活性	1. Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.37ng/ml. (Regularly tested) 2. Immobilized Human TGFBR2-Fc at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi . The ED50 of Biotinylated Human TGF-beta 3-Avi is 2.05 ng/ml. (Regularly tested)
产品描述	TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.

种属	Human
表达系统	HEK293 Cells
标签	N-Avi
蛋白编号	P10600
别名	ARVD, TGF-β3, TGFB3, 转化生长因子, TGF-beta3
氨基酸序列	Ala301-Ser412
蛋白构建	Ala301-Ser412 (Tyr340Phe)
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	10-14 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 4 mM HCl.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation.

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TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

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