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TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

产品编号 TMPJ-00255

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

产品编号 TMPJ-00255
TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.
规格价格库存数量
20 μg
¥ 5,730
5日内发货
100 μg
¥ 15,800
5日内发货
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常见问题解答
产品的溶解度是多少?
建议您先查询TargetMol官网、陶术官网,看该抑制剂的产品介绍,找到溶解度信息。如果没有相应信息,建议联系技术对该化合物的溶解度进行测定。
抑制剂能否用于细胞实验?
可以的,我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思?
这句话指的是,如果溶解度 < 1 mg/mL,那我们就认为它是微溶或不溶的,这是针对写的“DMSO:Insoluble”或“DMSO:slightly soluble”这类化合物的。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
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产品信息

生物活性
1. Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.37ng/ml. (Regularly tested) 2. Immobilized Human TGFBR2-Fc at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi . The ED50 of Biotinylated Human TGF-beta 3-Avi is 2.05 ng/ml. (Regularly tested)
产品描述
TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.
种属
Human
表达系统
HEK293 Cells
标签N-Avi
蛋白编号P10600
别名
转化生长因子,TGF-β3,TGF-beta3,TGFB3,ARVD
氨基酸序列
Ala301-­Ser412
蛋白构建
Ala301-­Ser412 (Tyr340Phe)
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量10-14 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 4 mM HCl.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation.

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