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TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated

产品编号 TMPJ-00255
别名: ARVD, TGF-β3, TGFB3, 转化生长因子, TGF-beta3

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.

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TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated
规格 价格/CNY 货期 数量
20 μg ¥ 5,730 5日内发货
100 μg ¥ 15,800 5日内发货
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产品目录号及名称: TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated (TMPJ-00255)
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生物活性
技术参数
产品性质
生物活性 1. Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.37ng/ml. (Regularly tested) 2. Immobilized Human TGFBR2-Fc at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi . The ED50 of Biotinylated Human TGF-beta 3-Avi is 2.05 ng/ml. (Regularly tested)
产品描述 TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.
种属 Human
表达系统 HEK293 Cells
标签 N-Avi
蛋白编号 P10600
别名 ARVD, TGF-β3, TGFB3, 转化生长因子, TGF-beta3
氨基酸序列 Ala301-­Ser412
蛋白构建 Ala301-­Ser412 (Tyr340Phe)
蛋白纯度 Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量 10-14 KDa (reducing condition)
内毒素 < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 4 mM HCl.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation.

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Keywords

TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated ARVD TGFB-3 TGFB 3 TGF-β3 TGFB3 转化生长因子 TGF-beta3 recombinant recombinant-proteins proteins protein

 

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