TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 5,730 | 5日内发货 | |
| 100 μg | ¥ 15,800 | 5日内发货 |
| 生物活性 | 1. Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.37ng/ml. (Regularly tested)
2. Immobilized Human TGFBR2-Fc at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi . The ED50 of Biotinylated Human TGF-beta 3-Avi is 2.05 ng/ml. (Regularly tested) |
| 产品描述 | TGF beta 3 Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with N-Avi tag. The predicted molecular weight is 10-14 KDa and the accession number is P10600. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | N-Avi |
| 蛋白编号 | P10600 |
| 别名 | 转化生长因子,TGF-β3,TGF-beta3,TGFB3,ARVD |
| 氨基酸序列 | Ala301-Ser412 |
| 蛋白构建 | Ala301-Ser412 (Tyr340Phe) |
| 蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 分子量 | 10-14 KDa (reducing condition) |
| 内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 4 mM HCl. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation. |
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