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TGF alpha Protein, Human, Recombinant (hFc)

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货号 TMPK-00757

别名 转化生长因子, TGF-α, TGF-alpha, TGFA, TGF type 1, ETGF

Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice. TGF alpha Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 32.9 kDa and the accession number is P01135-1.

TGF alpha Protein, Human, Recombinant (hFc)
其他形式的 “TGF alpha Protein, Human, Recombinant (hFc)”:
TGF alpha Protein, Mouse, Recombinant (hFc)
TGF alpha Protein, Human, Recombinant

TGF alpha Protein, Human, Recombinant (hFc)

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Rating icon 还可以
货号 TMPK-00757 别名 转化生长因子, TGF-α, TGF-alpha, TGFA, TGF type 1, ETGF

Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice. TGF alpha Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 32.9 kDa and the accession number is P01135-1.

规格价格库存数量
5 μg
¥ 358
6-8日内发货
10 μg
¥ 573
6-8日内发货
20 μg
¥ 938
5日内发货
50 μg
¥ 1,830
5日内发货
100 μg
¥ 3,100
5日内发货
200 μg
¥ 5,620
5日内发货
500 μg
¥ 12,400
5日内发货
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产品介绍

生物活性
产品描述
Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice. TGF alpha Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 32.9 kDa and the accession number is P01135-1.
生物活性
1. Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test). 2. Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay.
研究背景
Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice.
种属
Human
表达系统
HEK293 Cells
标签N-hFc
蛋白编号P01135-1
蛋白构建Val40-Ala89
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl (pH 8.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名转化生长因子, TGF-α, TGF-alpha, TGFA, TGF type 1, ETGF
内毒素< 1 EU/μg by the LAL method.
化学信息
分子量32.9 kDa (predicted). Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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技术支持

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