The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 100.6 kDa and the accession number is P59594.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 11,800 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | Immobilized SARS Spike S1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21μg/ml determined by ELISA. |
产品描述 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 100.6 kDa and the accession number is P59594. |
种属 | SARS |
表达系统 | HEK293 Cells |
标签 | C-hFc-Avi |
蛋白编号 | P59594 |
别名 | Spike protein S1, S1 protein, Spike,S1 protein, S glycoprotein Subunit1 |
蛋白构建 | Ser14-Arg667 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 100.6 kDa (predicted). Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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SARS Spike S1 Protein (hFc & Avi) Spike protein S1 S1 protein Spike,S1 protein S glycoprotein Subunit1 recombinant recombinant-proteins proteins protein