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SARS Spike S1 Protein (hFc & Avi)

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产品编号 TMPK-00908

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 100.6 kDa and the accession number is P59594.

SARS Spike S1 Protein (hFc & Avi)

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产品编号 TMPK-00908

规格	价格	库存
5 μg	¥ 343	6-8日内发货
10 μg	¥ 549	6-8日内发货
20 μg	¥ 898	5日内发货
50 μg	¥ 1,760	5日内发货
100 μg	¥ 2,960	5日内发货
200 μg	¥ 5,350	5日内发货
500 μg	¥ 11,800	5日内发货

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产品信息

生物活性	Immobilized SARS Spike S1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21μg/ml determined by ELISA.
产品描述	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 100.6 kDa and the accession number is P59594.
种属	SARS
表达系统	HEK293 Cells
标签	C-hFc-Avi
蛋白编号	P59594
别名	Spike,S1 protein,Spike protein S1,S1 protein,S glycoprotein Subunit1
蛋白构建	Ser14-Arg667
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	100.6 kDa (predicted). Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.