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SARS-COV-2 Spike S1 Protein (hFc & Avi)

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SPR兼容缓冲液
货号 TMPK-00432

别名 Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 102.6 kDa and the accession number is A0A6G7K2L4.

SARS-COV-2 Spike S1 Protein (hFc & Avi)

SARS-COV-2 Spike S1 Protein (hFc & Avi)

一键复制产品信息
Rating icon 很棒
SPR兼容缓冲液
货号 TMPK-00432 别名 Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 102.6 kDa and the accession number is A0A6G7K2L4.

规格价格库存数量
5 μg
¥ 343
现货
10 μg
¥ 549
现货
20 μg
¥ 898
现货
50 μg
¥ 1,760
现货
100 μg
¥ 2,960
现货
200 μg
¥ 5,350
5日内发货
500 μg
¥ 11,800
5日内发货
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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产品介绍


生物活性
产品描述
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 Protein (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 102.6 kDa and the accession number is A0A6G7K2L4.
生物活性
1. Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA. 2. SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay.
研究背景
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
种属
SARS-CoV-2
表达系统
HEK293 Cells
标签C-hFc-Avi
蛋白编号A0A6G7K2L4
蛋白构建Gln14-Arg683
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1
内毒素< 1 EU/μg by the LAL method.
化学信息
分子量102.6 kDa (predicted). Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.
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