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Siglec-2/CD22 Protein, Human, Recombinant (hFc)

Siglec-2/CD22 Protein, Human, Recombinant (hFc)

产品编号 TMPJ-00393
别名: BL-CAM, SIGLEC2FLJ22814, CD22 antigenMGC130020, sialic acid binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule, T-cell surface antigen Leu-14, Siglec-2, CD22, B-cell receptor CD22, CD22 molecule

Siglec-2/CD22 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 120-150 KDa and the accession number is P20273.

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Siglec-2/CD22 Protein, Human, Recombinant (hFc)
规格 价格/CNY 货期 数量
10 μg ¥ 820 5日内发货
50 μg ¥ 2,480 5日内发货
500 μg ¥ 12,100 5日内发货
1 mg ¥ 17,400 5日内发货
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产品目录号及名称: Siglec-2/CD22 Protein, Human, Recombinant (hFc) (TMPJ-00393)
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生物活性
技术参数
产品性质
生物活性 Immobilized Anti-Human Siglec-2 mAb at 2μg/ml (100 μl/well)can bind Human Siglec-2-Fc. The ED50 of Human Siglec-2-Fc is 24.05 ng/ml. (Regularly tested)
产品描述 Siglec-2/CD22 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 120-150 KDa and the accession number is P20273.
种属 Human
表达系统 HEK293 Cells
标签 C-hFc
蛋白编号 P20273
别名 BL-CAM, SIGLEC2FLJ22814, CD22 antigenMGC130020, sialic acid binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule, T-cell surface antigen Leu-14, Siglec-2, CD22, B-cell receptor CD22, CD22 molecule
氨基酸序列 Asp20-Arg687
蛋白构建 Asp20-Arg687
蛋白纯度 Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量 120-150 KDa (reducing condition)
内毒素 < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, pH 8.0.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

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Keywords

Siglec-2/CD22 Protein, Human, Recombinant (hFc) CD-22 CD22 antigenMGC-130020 CD22 antigenMGC 130020 BL-CAM SIGLEC2FLJ22814 SIGLEC2FLJ-22814 CD22 antigenMGC130020 sialic acid binding Ig-like lectin 2 B-lymphocyte cell adhesion molecule T-cell surface antigen Leu-14 Siglec-2 CD 22 CD22 SIGLEC2FLJ 22814 B-cell receptor CD22 CD22 molecule recombinant recombinant-proteins proteins protein

 

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