Name: SHP-2 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03125
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

5 μg

¥ 385

6-8日内发货

10 μg

¥ 623

6-8日内发货

20 μg

¥ 987

5日内发货

50 μg

¥ 1,980

5日内发货

100 μg

¥ 3,380

5日内发货

200 μg

¥ 6,670

5日内发货

生物活性

产品描述	SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.
生物活性	Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site . The specific activity is > 1 pmoles/min/μg.
研究背景	SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P35235-2
蛋白构建	A DNA sequence encoding the mouse PTPN11 (P35235-2) (Met1-Arg593) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Met
蛋白纯度	> 90 % as determined by SDS-PAGE
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.1M NaCl, 10% Glycerol, 1 mM TCEP, pH 7.8. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名	Syp, SH-PTP3, SH-PTP2, SHP-2, Shp2, SAP-2, PTP2C, PTP1D, protein tyrosine phosphatase, non-receptor type 11, AW536184, 2700084A17Rik
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	69.5 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式	In general, Lyophilized powders are shipping with blue ice.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

SHP-2 Protein, Mouse, Recombinant (His)

SHP-2 Protein, Mouse, Recombinant (His)

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