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SARS-CoV-2 Spike S1 Protein (His), Biotinylated

产品编号 TMPY-05694

SARS-CoV-2 Spike S1 Protein (His), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.5 kDa and the accession number is A0A6G7K2L4.

SARS-CoV-2 Spike S1 Protein (His), Biotinylated

SARS-CoV-2 Spike S1 Protein (His), Biotinylated

产品编号 TMPY-05694
SARS-CoV-2 Spike S1 Protein (His), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.5 kDa and the accession number is A0A6G7K2L4.
规格价格库存数量
20 μg
¥ 4,460
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产品信息

生物活性
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1, His tag), Biotinylated , the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1, His tag), Biotinylated is 200-600 ng/mL.
产品描述
SARS-CoV-2 Spike S1 Protein (His), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.5 kDa and the accession number is A0A6G7K2L4.
种属
SARS-CoV-2
表达系统
HEK293 Cells
标签C-His
蛋白编号A0A6G7K2L4
蛋白构建
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Val16
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量76.5 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

参考文献

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