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SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated

产品编号 TMPY-06267

SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 78.09 kDa and the accession number is A0A6G7K2L4.

SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated

SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated

产品编号 TMPY-06267
SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 78.09 kDa and the accession number is A0A6G7K2L4.
规格价格库存数量
20 μg
¥ 4,460
5日内发货
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常见问题解答
小规格产品,收到瓶子后看不到任何东西?
由于产品规格小,粉末在运输过程中产生的静电导致过于分散,管壁和盖子上皆会粘着,您可以放心,离心后加入适量溶剂直接配制母液、充分溶解即可。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
溶解度写的“mg/mL”和“mM”是什么意思?
mg/mL 与 mM,是两种不同单位的表示方式,所指代的最大溶解度是一样的,可以通过官网网页下方的摩尔浓度计算器进行换算
抑制剂产品应如何储存?
粉末储存在 -20℃,可以存 3 年以上。 母液一般储存于 -80℃,可以存 1 年以上,建议多分装几管,避免反复冻融。 常用的存于 4℃,可放一周以上。
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
SARS-CoV-2 Spike S1 Protein (157-158 deletion & T19R & G142D & E156G & L452R & T478K & D614G & P681R, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 78.09 kDa and the accession number is A0A6G7K2L4.
种属
SARS-CoV-2
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号A0A6G7K2L4
蛋白构建
A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations (157-158) deletion, T19R, G142D, E156G, L452R, T478K, D614G, P681R) (Met1-Arg685) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India. Predicted N terminal: Val 16
蛋白纯度
> 95 % as determined by SDS-PAGE.
分子量78.09 kDa (predicted); 85.61 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

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