R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 28.5 kDa and the accession number is B1ASC1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,930 | 现货 | ||
1 mg | ¥ 25,600 | 5日内发货 |
生物活性 | Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/ml in the presence of 5 ng/mL recombinant mouse Wnt3a. |
产品描述 | R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 28.5 kDa and the accession number is B1ASC1. |
种属 | Mouse |
表达系统 | CHO Cells |
标签 | C-His |
蛋白编号 | B1ASC1 |
别名 | R-spondin, R-spondin 1, Rspondin |
蛋白构建 | The full length of Mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 28.5 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6. |
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R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) R-spondin R-spondin 1 Rspondin recombinant recombinant-proteins proteins protein