生物活性

产品描述	Peptide Ready HLA-A33:03&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is VCU43110.1(HLA-A33:03)&P61769(B2M).
生物活性	Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test).
研究背景	HLA-A33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	VCU431101 (HLA-A*33:03) & P61769 (B2M)
蛋白构建	Gly25-Thr305 (HLA-A*33:03) and Ile21-Met119 (B2M)
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	48.3 kDa (Predicted); 50-65 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

**Peptide Ready HLA-A*33:03&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated**

Peptide Ready HLA-A*33:03&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated

产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.

产品介绍

引用文献

计算器

剂量转换

技术支持