PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,210 | 5日内发货 | ||
500 μg | ¥ 8,900 | 5日内发货 | ||
1 mg | ¥ 14,800 | 5日内发货 |
生物活性 | Immobilized Human PDGF R beta, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. |
产品描述 | PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P09619 |
别名 | PDGFR1, CD140B, PDGFR-2, PDGF R β/CD140b, PDGF R beta, PDGFRB, PDGFR-β, JTK12, PDGFR, PDGF R β |
蛋白构建 | Leu33-Phe530 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 59 kDa (predicted). Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
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PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) PDGFR1 JTK 12 CD140B PDGFR2 PDGFR-1 PDGFR-2 PDGFR-beta JTK-12 PDGF R β/CD140b PDGF R beta PDGFRB PDGFR-β PDGF R b JTK12 PDGFR PDGF R β PDGFR-b PDGFR 2 PDGFR 1 recombinant recombinant-proteins proteins protein