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PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-00421
PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619.
规格价格库存数量
100 μg
¥ 2,210
5日内发货
500 μg
¥ 8,900
5日内发货
1 mg
¥ 14,800
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
期货产品没有纯度啊?这个纯度在哪里看
期货产品的话,是没有纯度信息,也没有质检信息的。有了现货之后我们会进行质检,测出试剂的纯度和结构等相关信息,并且展示在官网的活性描述上方。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
抑制剂母液的储存方式?
母液配置完成后,一般储存于 -80℃,可以存 1 年以上。建议多分装几管,避免反复冻融,常用的存于 4 度,可放一周以上。
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产品信息

生物活性
Immobilized Human PDGF R beta, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.
产品描述
PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号P09619
别名
PDGFR-β,PDGFRB,PDGFR-2,PDGFR1,PDGFR,PDGF R β/CD140b,PDGF R β,PDGF R beta,JTK12,CD140B
蛋白构建
Leu33-Phe530
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量59 kDa (predicted). Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.

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