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LEPR Protein, Mouse, Recombinant (mFc)

产品编号 TMPJ-00977

LEPR Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 130-190 KDa and the accession number is P48356.

LEPR Protein, Mouse, Recombinant (mFc)

LEPR Protein, Mouse, Recombinant (mFc)

产品编号 TMPJ-00977
LEPR Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 130-190 KDa and the accession number is P48356.
规格价格库存数量
10 μg
¥ 820
5日内发货
50 μg
¥ 2,480
5日内发货
500 μg
¥ 12,400
5日内发货
1 mg
¥ 18,700
5日内发货
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实验操作小课堂
常见问题解答
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思?
这句话指的是,如果溶解度 < 1 mg/mL,那我们就认为它是微溶或不溶的,这是针对写的“DMSO:Insoluble”或“DMSO:slightly soluble”这类化合物的。
动物实验常用溶解方法?
首先,您需要确认给药剂量、给药方式。对于具体的产品,优先找引用我们产品的文献里的使用方法,再找其他文献里的配方。 如果没有相关的文献,且该化合物的 DMSO 溶解度比较好,我们推荐通用配方: 10% DMSO+40% PEG300+5% Tween-80+45% Saline/PBS/ddH2O。溶剂依次加入,尽量溶解后再加入下一个溶剂。正常鼠建议 DMSO 浓度在 10% 以下,裸鼠体弱鼠等配方的 DMSO 浓度建议在 2% 以下,根据溶液是否澄清,助溶剂 PEG300、Tween-80 的比例可以适当调整。如果有其他助溶剂也可以使用。 以上配方仅供参考,体内配方并不是绝对的,需要根据不同情况进行调整。建议先取少量化合物测试配方,再进行大量配制。配完也可以再使用超声、加热等方式助溶,看看能不能澄清一点。 腹腔注射对粉末的溶解度要求比较高,建议购买盐形式化合物。如果给药剂量比较大,也有报道使用混悬液进行腹腔给药的。 对于灌胃给药,且剂量比较大的情况下,建议用 0.5% CMC-Na 配置成均匀混悬液进行给药。
如何选择合适的溶剂?
常用的溶剂包括DMSO、水、乙醇等。建议您根据抑制剂给出的溶解度参考,选择合适的溶剂。如果该抑制剂可以溶于DMSO,建议使用没有吸潮新开封的 DMSO,如果有潮气污染,很容易带来抑制剂降解或难溶的问题。如果该抑制剂可以溶于水,建议您根据实验类型选择无菌水、生理盐水、无菌 PBS 、培养基等作为溶剂。
溶解度写的“mg/mL”和“mM”是什么意思?
mg/mL 与 mM,是两种不同单位的表示方式,所指代的最大溶解度是一样的,可以通过官网网页下方的摩尔浓度计算器进行换算
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
LEPR Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 130-190 KDa and the accession number is P48356.
种属
Mouse
表达系统
HEK293 Cells
标签C-mFc
蛋白编号P48356
别名
OB-R,OBR,OB receptor,Leptin receptor,LEP-R,LEPR,HuB219,DB,CD295
氨基酸序列
Leu22­Gly839
蛋白构建
Leu22­Gly839
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量130-190 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
The Leptin receptor is a member of the Class I cytokine receptor family. It mediates the activities of Leptin, a multi-functional hormone produced primarily by adipose tissues that plays roles in food intake, energy metabolism, angiogenesis, reproduction, hematopoiesis, bone metabolism, and immune function. The human Leptin R gene encodes 1165 amino acids (aa) including a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain that supports JAK/STAT signaling. Soluble Leptin R is the primary Leptin-binding protein in blood, where it maintains a pool of available bioactive Leptin, delays Leptin clearance from circulation, and down-regulates blood-brain transmission of Leptin. In humans, soluble Leptin R levels are inversely proportional to adiposity and are elevated in females versus males. Soluble Leptin R is also found up-regulated in patients with chronic heart failure, end-stage renal disease, and anorexia.It is expressed by tumor-initiating stem cells, and is proposed as a link between cancer and obesity.

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